Ribonucleic acid polymerase activity as a measure of ribonucleic acid synthesis
نویسندگان
چکیده
منابع مشابه
Enzymatic Synthesis of Ribonucleic Acid. I. Preparation of Ribonucleic Acid Polymerase from Extracts of Micrococcus Lysodeikticus.
The problem of complex polynucleotide synthesis has received considerable attention from numerous investigators (l-3). Grunberg-Manago and Ochoa (4) and Kornberg, Lehman, and Simms (5) first successfully demonstrated the polymerization in vitro of riboand deoxynucleotides with cell-free extracts from microorganisms. Subsequent studies on the synthesis of ribonucleic acid and deoxyribonucleic ac...
متن کاملThe Enzymatic Synthesis of Ribonucleic Acid. v. the Interaction of Ribonucleic Acid Polymerase with Nucleic Acids.
Numerous reports have been published on the properties and reaction mechanism of the highly purified enzyme preparations that catalyze the deoxyribonucleic acid-directed synthesis of ribonucleic acid (l-6). The various RNA polymerases tudied, although differing in some respects, show a remarkable similarity in their cofactor requirements, reaction kinetics, physical properties, and facility to ...
متن کاملEnzymatic Synthesis of Ribonucleic Acid 1. PREPARATION OF RIBONUCLEIC ACID POLYMERASE FROM EXTRACTS OF MICROCOCCUS LYSODEIKTICUP
The problem of complex polynucleotide synthesis has received considerable attention from numerous investigators (l-3). Grunberg-Manago and Ochoa (4) and Kornberg, Lehman, and Simms (5) first successfully demonstrated the polymerization in vitro of riboand deoxynucleotides with cell-free extracts from microorganisms. Subsequent studies on the synthesis of ribonucleic acid and deoxyribonucleic ac...
متن کاملAzotobacter vinehndii Ribonucleic Acid Polymerase
RNA synthesis by the Azofobacter vinelandii RNA polymerase is initiated with the incorporation of a ribonucleoside triphosphate to form the 5’ terminus of the chain. The RNA chains synthesized in vifro are preferentially initiated by purine ribonucleoside triphosphates. The ratio of guanosine triphosphate to ATP termini is determined by the DNA used to direct the synthesis of RNA. When denature...
متن کاملEnzymatic Synthesis of Ribonucleic Acid. Ii. Properties of the Deoxyribonucleic Acid-primed Reaction with Micrococcus Lysodeikticus Ribonucleic Acid Polymerase.
The discovery of enzymes which catalyze the deoxyribonucleic acid-dependent synthesis of ribopolynucleotides (l-6) stimulated an immediate inquiry into the role played by DNA in thisunique reaction. The speculation that DNA might serve as a template for the polymerization of ribonucleotides into a complementary ribonucleic acid molecule was confirmed by studies from a number of different groups...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj1250074pa